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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Curr Opin Biotechnol. 2022 Mar 9;75:102701. doi: 10.1016/j.copbio.2022.102701

Figure 3. Analytical toolbox for absolute metabolic flux quantification.

Figure 3.

Each tool provides different information that can be integrated to calculate absolute metabolic fluxes and determine a full picture of metabolism. (a) Table comparing the strengths and weaknesses of 11 commonly used and emerging analytical tools. The scope relates to the breadth of analytes that can be measured, which in turn reflects the number of fluxes that can be quantified. (b) Venn diagram showing the types of information provided by each tool. DIMS, direct-injection mass spectrometry; and 13C MRI, hyperpolarized 13C magnetic resonance imaging.