Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli BL21 (DE3) | Nippon Gene | 312-06534 |
| Chemicals, peptides, and recombinant proteins | ||
| LB Broth with agar (Miller) | Sigma-Aldrich | L3147 |
| Kanamycin sulfate | Fujifilm Wako | 113-00343 |
| LB Broth (Miller) | Sigma-Aldrich | L3522 |
| Isopropyl-β-D-thiogalactoside | Sigma-Aldrich | I5502 |
| d-myo-phosphatidylinositol 5-phosphate | Echelon Biosciences | P-0016d |
| 1,2-dipalmitoyl-phosphatidylserine | Echelon Biosciences | L-3116 |
| GTP | TriLink BioTechnologies | N-1512 |
| ATP | TriLink BioTechnologies | N-1510 |
| ITP | TriLink BioTechnologies | N-4017 |
| XTP | TriLink BioTechnologies | N-1023 |
| 6-thio-GTP | TriLink BioTechnologies | N-8007 |
| 6-OMe-GTP | TriLink BioTechnologies | N-1031 |
| 2a-6Cl-PNP | TriLink BioTechnologies | N-1002 |
| 2a-ATP | TriLink BioTechnologies | N-1001 |
| 6Cl-PNP | TriLink BioTechnologies | N-2009 |
| 2-oxo-ATP | TriLink BioTechnologies | N-1099 |
| Deposited data | ||
| Crystal structure of WT-GMPPNP complex | This Work | 6K4G |
| Crystal structure of WT-AMPPNP complex | This Work | 6K4H |
| Crystal structure of WT-ITP complex | This Work | 7EM1 |
| Crystal structure of WT-XTP complex | This Work | 7EM2 |
| Crystal structure of WT-2a-ATP complex | This Work | 7EM3 |
| Crystal structure of F205L-ITP complex | This Work | 7EM4 |
| Crystal structure of F205L-XTP complex | This Work | 7EM5 |
| Crystal structure of N203D-ITP complex | This Work | 7EM6 |
| Crystal structure of N203D-XTP complex | This Work | 7EM7 |
| Crystal structure of T201M-2a-ATP complex | This Work | 7EM8 |
| Oligonucleotides | ||
| T201M-f 5'-ATGGTGGTTATGAGGAACGTGTTC-3’ | Eurofins | N/A |
| T201M-r 5’-CACGTTCCTCATAACCACCATGTA-3’ | Eurofins | N/A |
| N203D-f 5’-GGTGGTTACCAGGGACGTGTTCAGCCATC-3’ | Eurofins | N/A |
| N203D-r 5’-GATGGCTGAACACGTCCCTGGTAACCACC-3’ | Eurofins | N/A |
| N203A-f 5’-CATGGTGGTTACCAGGGCGGTGTTCAGCCATCGG-3’ | Eurofins | N/A |
| N203A-r 5’-CCGATGGCTGAACACCGCCCTGGTAACCACCATG-3’ | Eurofins | N/A |
| F205L-f 5'-CCAGGAACGTGTTGAGCCATCGG-3’ | Eurofins | N/A |
| F205L-r 5’-ATGGCTCAACACGTTCCTGGTAACC-3’ | Eurofins | N/A |
| Software and algorithms | ||
| Pymol (Version 2.4.0) | Schrödinger | https://pymol.org/ |
| Topspin | Bruker | https://www.bruker.com/en/products-and-solutions/mr/nmr-software.html |
| Origin 2020 | Origin Lab Corporation | https://www.originlab.com/ |
| XDS | (Kabsch, 2010) | https://xds.mr.mpg.de/ |
| XSCALE | (Kabsch, 2010) | https://xds.mr.mpg.de/ |
| PHENIX | (Liebschner et al., 2019) | https://phenix-online.org/ |
| phenix.refine | (Liebschner et al., 2019) | https://phenix-online.org/ |
| PyMOL | Schrödinger, LLC. | https://pymol.org/2/ |
| Molecular Operating Environment | Chemical Computing Group, | https://www.chemcomp.com/Products.htm |
| ABINIT-MP | (Mochizuki et al., 2019; Nakano et al., 2006) | https://www.hpci-office.jp/pages/e_appli_abinit-mp |
| L-INS-i | MAFFT version 7.205 | https://mafft.cbrc.jp/alignment/software/ |
| TranslatorX | (Abascal et al., 2010) | https://translatorx.org/ |
| Single-Likelihood Ancestor Counting (SLAC) | Datamonkey | http://www.datamonkey.org/ |