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. 2021 Dec 1;29(6):1176–1186. doi: 10.1038/s41418-021-00908-7

Fig. 3. The attenuated sensitivity to TNF-induced cell death in Bcl-3-deficient mice relies on caspase activity.

Fig. 3

A qPCR analysis of NF-κB target genes associated with survival and apoptosis. B Cell lysates were prepared from liver tissues 6 h post injection and probed by immunoblotting. C qPCR analysis of inflammation markers and immune cell infiltration markers in either WT or KO liver tissues with T + D injection. D qPCR analysis of inflammation markers and immune cell infiltration markers in liver tissues from mice with 20 mg/kg sp600125 pretreatment followed by T + D injection. E Survival rates of WT and KO mice with T + D or T + D + S treatment. F Survival rates of WT and KO mice with or without 10 mg/kg Z-VAD-FMK pretreatment followed by T + D injection. G Western blot analysis of cleaved Caspase 3 and PARP in liver tissues from mice with indicated treatment at 10 h. H Bcl-3 overexpressing and control MEFs were photographed by phase contrast microscopy after pretreatment with or without 20 μM Z-VAD-FMK as indicated (scale bar = 50 μm). I Western blot analysis of caspase and JNK activity as in (H). J Bcl-3 overexpressing and control MEFs were photographed after pretreatment with or without 20 μM sp600125 followed by T + C treatment as indicated (scale bar = 50 μm). K Western blot analysis of caspase and JNK activity as indicated. The results are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations are as follows: T + D + S, TNF + D-GalN+sp600125; T + D + Z, TNF + D-GalN+Z-VAD-FMK; T + C, TNF (10 ng/ml) +CHX (10 μg/ml); T + C + Z, TNF + CHX + Z-VAD-FMK; T + C + S, TNF + CHX + sp600125.