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. 2021 Dec 1;29(6):1176–1186. doi: 10.1038/s41418-021-00908-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Bcl-3-knockdown efficiency in tet-on stable LO2 cells upon DOX treatment was quantified by qPCR and western blotting. B Bcl-3 knockdown efficiency was analyzed in tet-on stable HepG2 cells upon DOX treatment. C LO2-shBcl-3 cells were treated with the indicated stimulations and then photographed (scale bar = 50 μm) and analyzed by flow cytometry for Annexin V/7-AAD staining. D HepG2-shBcl-3 cells were treated with the indicated stimulations and then photographed (scale bar = 50 μm) and analyzed by flow cytometry for Annexin V/7-AAD staining. E Western blot analysis of caspase and JNK activation in Bcl-3 knockdown LO2 cells with T + C treatment. F Western blot analysis of caspase and JNK activation in Bcl-3 knockdown HepG2 cells with T + C treatment. G LO2-shBcl-3 cells were treated with or without Z-VAD-FMK and analyzed by western blot. H HepG2-shBcl-3 cells were treated with or without Z-VAD-FMK and analyzed by western blot. The results are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.