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. Author manuscript; available in PMC: 2022 Dec 3.
Published in final edited form as: Mol Cancer Res. 2022 Jun 3;20(6):960–971. doi: 10.1158/1541-7786.MCR-21-0604

Figure 2. RNA-seq analysis identifies ADAR1-regulated pathways.

Figure 2.

A) Volcano plot for high-confidence DE poly(A)+ RNAs, with ADAR1 noted. B) Enrichment results for siADAR-upregulated genes using GSEA on KEGG terms. Normalized enrichment score (NES) and −log10(FDR) are shown in blue and red, respectively. FDR cutoffs are indicated. C) qPCR for IL6/TNF in 72hr ADAR1-KD BT20s. D) ELISA for IL6 in 72hr ADAR1-KD BT20s. E) Enrichment results for siADAR-downregulated genes using GSEA on KEGG terms. Normalized enrichment score (NES) and −log10(FDR) are shown in blue and red, respectively. FDR cutoffs are indicated. F) Volcano plot for high-confidence DE miRNAs, with miRNAs 27a-5p and 4485-3p indicated and miRNAs with previous connection to ADAR1 highlighted in red (see Table S5). G) Enrichment results for TargetScan predicted targets of all siADAR-upregulated miRNAs using GSEA on Hallmark terms. Normalized enrichment score (NES) and −log10(FDR) are shown in blue and red, respectively. FDR cutoffs are indicated.