Figure 1. Binding characteristics of new anti-CD123 mAbs.
A panel of anti-CD123 mAbs (11F11, 10C4, 7G3, 5G4, 1H8; used at 2 μg/mL) was tested flow cytometrically against parental RS4;11 cells (endogenously lacking CD123), RS4;11 cells transduced with human CD123, CD123-expressing human AML cells lines (TF-1, KG-1, MOLM-13), and MOLM-13 cells with CRISPR/Cas9-mediated knockout of the CD123 locus (CD123KO). Secondary antibody only negative control is shown as well.