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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Hypertension. 2022 May 5;79(7):1536–1547. doi: 10.1161/HYPERTENSIONAHA.121.18832

Figure 6. Leptin increases placental endothelin-1 production and increases adrenal CYP11B2 and AT1Rb expression in pregnant mice.

Figure 6.

Placental mRNA expression of prepro-endothelin-1 (PPET-1) (A) and endothelin converting enzyme-1 (ECE-1) increased with leptin infusion in WT pregnant mice (B). Adrenal protein expression of CYP11B2 increased in WT mice infused with leptin (C). Adrenal angiotensin II type 1 receptor a (AT1Ra) mRNA expression did not increase with leptin infusion in WT pregnant mice (D) in contrast to the leptin-induced increase in AT1Rb observed in leptin-infused WT pregnant mice (E). Placental N values: N=7 WT+Vehicle, N=9 WT+Leptin, N=4 KO+Vehicle, N=4 KO+Leptin. Protein expression data N=4 for all groups. Adrenal N values: N=5 WT+Vehicle, N=4 WT+Leptin, N=5 KO+Vehicle, N=4 KO+Leptin. Student’s unpaired t-test for paired comparisons to respective Vehicle, 2-way ANOVA with Tukey’s posthoc test for multiple comparisons for protein expression. *P<0.05.