KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-FOS | Santa Cruz Biotechnology | Cat# sc-166940; RRID:AB_10609634 |
| Rabbit anti-α-tubulin | Abcam | Cat# ab18251; RRID:AB_2210057 |
| Rat anti-α-tubulin | Abcam | Cat# ab6161; RRID:AB_305329 |
| Rabbit anti-α-tubulin | GeneTex | Cat# GTX628802; RRID:AB_2716636 |
| Mouse anti-γ-tubulin | Abcam | Cat# ab11316; RRID:AB_297920 |
| Mouse anti-MYOD | BD Biosciences | Cat# 554130; RRID:AB_395255 |
| Rabbit anti-Ddx6 | Bethyl Laboratories | Cat# A300-461A; RRID:AB_2277216 |
| Rabbit anti-MKL1 | ProteinTech | Cat# 21166-1-AP; RRID:AB_2878822 |
| Mouse anti-pericentrin | BD Biosciences | Cat# 611814; RRID:AB_399294 |
| Rabbit anti-Lamin B1 | Abcam | Cat# ab16048; RRID:AB_10107828 |
| Mouse anti-N-cadherin | Santa Cruz Biotechnology | Cat# sc-8424; RRID:AB_626778 |
| Mouse anti-Arp3 | Sigma-Aldrich | Cat# A5979; RRID:AB_476749 |
| Mouse anti-dystrophin | Santa Cruz Biotechnology | Cat# sc-73592; RRID:AB_1122390 |
| Mouse anti-β-catenin | BD Biosciences | Cat# 610154; RRID:AB_397555 |
| Rabbit anti-α-catenin | ProteinTech | Cat# 13974-1-AP; RRID:AB_2088078 |
| Mouse anti-M-cadherin | Santa Cruz Biotechnology | Cat# sc-81471; RRID:AB_2077111 |
| Mouse anti-p120ctn | BD Biosciences | Cat# 610133; RRID:AB_397536 |
| Mouse anti-Rac1 | BD Biosciences | Cat# 610650; RRID:AB_397977 |
| Mouse Alexa Fluor 647 preconjugated Mouse anti-Ncad antibody | Santa Cruz Biotechnology | Cat# sc-8424 AF647; RRID:AB_626778 |
| Mouse anti-Pax7-c | Developmental Studies Hybridoma Bank | Cat# PAX7; RRID:AB_2299243 |
| Rabbit anti-Laminin | Sigma-Aldrich | Cat# L9393; RRID:AB_477163 |
| Rabbit anti-Ki67 | Abcam | Cat# ab15580; RRID:AB_443209 |
| Hoescht | Cell Signaling Technology | Cat# 4082; RRID:AB_10626776 |
| Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate | Invitrogen | Cat# W11261 |
| Anti-glutathione S-transferase, Alexa 488 conjugate | Thermo Fisher Scientific | Cat# A-11131; RRID:AB_2534137 |
| Goat anti-mouse IgG1 cross-adsorbed secondary antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-21240; RRID:AB_2535809 |
| Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008; RRID:AB_143165 |
| Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-21245; RRID:AB_2535813 |
| Goat anti-mouse IgG2b cross-adsorbed secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21141; RRID:AB_2535778 |
| Goat anti-rat IgG2a secondary antibody, FITC | Thermo Fisher Scientific | Cat# PA1-84761; RRID:AB_933936 |
| Goat anti-mCherry antibody | SICGEN | Cat# AB0081-500; RRID:AB_2333095 |
| Alexa Fluor 568-conjugated Donkey anti-Goat antibody | Invitrogen | Cat# A-11057; RRID:AB_2534104 |
| Chicken anti-GFP antibody | Novus | Cat# NB100-1614; RRID:AB_10001164 |
| Goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 546 | Thermo Fisher Scientific | Cat# A-11003; RRID:AB_2534071 |
| Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11039; RRID:AB_2534096 |
| Mounting medium with DAPI | Abcam | Cat# ab104139 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| Barium Chloride | Sigma-Aldrich | Cat# 342920 |
| Collagenase Type I | Gibco | Cat# 17100-017 |
| Chick embryo extract | United States Biological Corporation | Cat# NC1202490 |
| ROCK inhibitor – Y-27632 | StemCell Technologies | Cat# 72304 |
| Myosin II inhibitor – Blebbistatin | Sigma-Aldrich | Cat# B0560 |
| Rac inhibitor – NSC23766 trihydrochloride | Sigma-Aldrich | Cat# SML0952 |
| Rho/MRTF/SRF inhibitor – CCG-203971 | Sigma-Aldrich | Cat# SML1422 |
| MEK1/2 inhibitor – PD 0325901 | Sigma-Aldrich | Cat# PZ0162 |
| Rhotekin-RBD protein | Cytoskeleton, Inc | Cat# RT01 |
| Pak1-PBD protein | Cytoskeleton, Inc | Cat# PAK01 |
| Histodenz buffer | Cosmo Bio USA | Cat# AXS-1002424 |
| Laminin | Roche | Cat# 11243217001 |
| Critical Commercial Assays | ||
| Satellite Cell Isolation Kit | Miltenyi Biotec | Cat# 130-104-268 |
| Anti-Integrin α-7 MicroBeads | Miltenyi Biotec | Cat# 130-104-261 |
| Experimental models: Organisms/strains | ||
| Mouse Pax7CreERT2 | IMSR | Cat# JAX:017763; RRID:IMSR_JAX:017763 |
| Mouse ROSA26LSL-TdTomato | IMSR | Cat# JAX:007914; RRID:IMSR_JAX:007914 |
| Mouse Rac1fl/fl | IMSR | Cat# JAX:005550; RRID:IMSR_JAX:005550 |
| Mouse Cdh2fl/fl | IMSR | IMSR Cat# JAX:007611, RRID:IMSR_JAX:007611 |
| Mouse Dmdmdx-4cv | IMSR | Cat# JAX:001801; RRID:IMSR_JAX:001801 |
| Mouse Tg:Pax7nGFP | From corresponding lab | Sambasivan et al., 2009 |
| Software and algorithms | ||
| Fiji | Open Source | RRID:SCR_002285; http://fiji.sc |
| Leica Application Suite | Licensed Software | RRID:SCR_016555; https://www.leica-microsystems.com/products/microscope-software/details/product/leica-application-suite/ |
| GraphPad Prism | Licensed Software | RRID:SCR_002798; http://www.graphpad.com/ |
| CellProfiler Image Analysis Software | Open Source | RRID:SCR_007358; http://cellprofiler.org |