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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Hypertension. 2022 May 4;79(7):1423–1434. doi: 10.1161/HYPERTENSIONAHA.122.19159

Figure 3. Dietary salt restriction leads to transient Na+-wasting in AS−/− mice.

Figure 3.

AS+/+ and AS−/− mice were acclimated to normal salt diet (NS: 0.49% NaCl and 0.8% K+) then treated with low salt diet (LS: 0.03% Na+ and 0.8% K+) for 5 days. A, Body weight was not significantly changed in AS−/− mice fed LS diet compared with AS+/+ mice. B and C, Na+ excretion was higher on LS Day 1 in AS−/− mice but was similar to AS+/+ mice on LS Day 5. D, K+ balance (K+ intake – urinary K+ excretion) was similar between AS−/− mice and AS+/+ mice. E, Plasma potassium trended higher on NS diet and was significantly higher on LS diet in AS−/− mice compared with AS+/+ mice. F, Plasma sodium levels did not change in AS−/− or AS+/+ mice. Data represent individual values and mean ± SEM. Statistical differences were examined by two-tailed unpaired t-tests (C and D), two-way repeated measures ANOVA (A and B), or two-way ANOVA, followed by post hoc Tukey tests (E and F). **, P < 0.01; ***, P < 0.001; N.S., P > 0.05.