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. 2022 Apr 7;30(6):721–729. doi: 10.1038/s41431-022-01095-w

Fig. 3. Expression and localization of N-DRC components in the human and mice sperm flagellum.

Fig. 3

A–F Immunofluorescence depicting different DRCs’ locations on the human sperm flagella. TCTE1/DRC5 was not detected in the patient sample (A). However, DRC1 (B), CCDC65/DRC2 (C), DRC3(D), GAS8/DRC4(E), and DRC7 (F) were detected. DRCs (red), acetylated-TUBULIN (green), and Hoechst (blue); scale bar = 10 μm. G Western Blot evaluation of mice sperm proteins. DRC1, CCDC65/DRC2, DRC3, GAS8/DRC4, and DRC7 were expressed, but not TCTE1/DRC5.