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. 2022 Jan 8;29(6):1137–1151. doi: 10.1038/s41418-021-00905-w

Fig. 2. WTAP facilitates NPC cell proliferation and metastasis in vitro.

Fig. 2

A GSEA of data from the GEO database (GSE12452) revealed that WTAP expression correlated positively with tumorigenesis and NPC carcinogenesis. B siRNA-mediated interference was used to knockdown WTAP in SUNE-1 and HONE-1 cells. Knockdown efficiency was determined using qRT-PCR (left panel) and western blotting (right panel) as indicated. C, D Cell proliferation was analyzed using CCK-8 (C) and colony formation (D) assays in SUNE-1 and HONE-1 cells with or without WTAP silencing. E Representative image (left panel) and statistical analysis (right panel) of Transwell migration and invasion assays in SUNE-1 and HONE-1 cells with or without WTAP knockdown. Scale bar: 200 μm. F Overexpress efficiency of WTAP was assessed using qRT-PCR (upper panel) and western blotting (lower panel). G, H Cell proliferation was analyzed using CCK-8 (G) and colony formation (H) assays in SUNE-1 and HONE-1 cells with or without WTAP overexpression. I Representative image (left panel) and statistical analysis (right panel) of Transwell migration and invasion assay in SUNE-1 and HONE-1 cells with WTAP overexpression. Scale bar: 200 μm. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01. The experiments were repeated at least three times independently.