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. 2022 Jun 8;13(6):536. doi: 10.1038/s41419-022-04959-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Levels of indicated proteins in five GBM stem cells derived from human GBM samples were determined via immunoblotting. B Protein levels of ITGB8 in GSCs and corresponding GSDCs were tested via immunoblotting. C Fluorescence staining for ITGB8 and Nestin in GSCs were evaluated. Scale bar = 25 μm. D Fluorescence staining for ITGB8 in GSCs and GSDCs were measured. Scale bar = 10/20 μm. E GSC#2 and GSC#4 fractionated into β8⁻ and β8⁺ cells, were cultured in serum-free medium to form floating spheres. Measurement of diameters was conducted in five randomly chosen neurospheres in each group. Scale bar = 100 μm. F Gene expression analysis of ITGB8 in GBM, LGG (TCGA) and normal brain tissues (GTEx) was conducted via GEPIA2 web tool. G Gene expression of ITGB8 in TCGA glioma dataset was analyzed based on WHO grade. H Kaplan–Meier analysis for overall survival of glioma patients with high or low ITGB8 expression in TCGA glioma datasets. I Correlation between gene expression of ITGB8 and stem cell markers in TCGA glioma dataset was evaluated. J Representative IHC images of Nestin and ITGB8 in human GBM tissues. Scale bar = 25/100 μm. Uncropped western blot images are shown in Supplementary Fig. 5. Data are expressed as mean ± SD.**p < 0.01, ***p < 0.001. IHC Immunohistochemistry.