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. 2022 May 25;50(10):5881–5898. doi: 10.1093/nar/gkac414

Figure 2.

Figure 2.

Purification of Sense and Antisense in vitro Transcribed LincRNA-p21 AluSx1 RNA. (A) depicts the size exclusion chromatogram of the sense and antisense AluSx1 RNA elution profile using the Superdex 200 Increase GL 10/300 column. SEC-MALS and SV-AUC experiments were performed with the fractions highlighted in red (sense) and blue (antisense). (B) shows the 10% urea PAGE gel used to ascertain the sense and antisense LincRNA-p21 RNA purity extracted using 0.5 mL fractions (volumes in red) using an ÄKTA Pure FPLC through a Superdex 200 Increase GL 10/300 SEC column. Fractions collected at 11.0 mL and 11.5 mL for sense and antisense AluSx1 purifications were consolidated and used for SAXS and SV-AUC experiments. A Quick-Load® Purple 100 bp DNA Ladder (NEB, Canada) was used for the 10% urea PAGE gels in lanes 1 and 7 of each gel. (C) dC/ds sedimentation coefficient distributions for sense (Red) and anti-sense (Blue) under 6M urea denaturing conditions. (D) same as (C), except transformed to molar mass distributions assuming a partial specific volume of 0.516 mL/g.