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. 2022 May 25;50(10):5881–5898. doi: 10.1093/nar/gkac414

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Small Angle X-Ray Scattering (SAXS) Characterisation of Sense (red) and Antisense (blue) LincRNA-p21 AluSx1 RNA. (A) merged scattering data of sense and antisense AluSx1 RNA depicting the scattering intensity (log I(q)) vs. scattering angle (q = 4πsinθ/λ). (B) Guinier plots allowing for the determination of R_g from the low-angle region data and representing the homogeneity of samples. (C) Dimensionless Kratky plots (I(q)/I(0)*(q*R_g)2 vs. q*R_g) of sense and antisense AluSx1 RNA depicting the elongated, tube-like structures because of the non-Gaussian, levelled-plateau shape of the curve. (D) Normalised pair distance distribution plots for sense and antisense AluSx1 RNA which permits the determination of R_g derived from the SAXS dataset and including each molecule's D_max.