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. 2022 May 12;50(10):5772–5792. doi: 10.1093/nar/gkac337

Figure 2.

Figure 2.

KHSRP depletion enhances axonal growth from naïve but not injury-conditioned DRG neurons. (A) Representative images of 24 h DRG neuron cultures from naïve and 7 days injury-conditioned Khsrp+/+ and Khsrp–/– mice immunostained for NF are shown [scale bar = 100 μm]. (B) Quantification of total axon length per neuron from cultures as in (A) shows significantly greater axon growth in Khsrp–/– compared to Khsrp+/+ DRGs. However, there is no significant difference in axon length between the genotypes when cultures were prepared 7 days after nerve crush injury (i.e. ‘injury-conditioned’ neurons). Data are expressed as mean ± SEM; refer to Supplemental Figure S2 for axon branching analyses (N ≥ 75 neurons analyzed per condition in three independent culture preparations; * P ≤ 0.05 and ** P ≤ 0.01 by one-way ANOVA with Tukey's post-hoc analysis). (C) Representative immunoblot shows signal for KHSRP in cell body and axonal preparations from DRG cultures from indicated mice. There is no KHSRP band in the Khsrp–/– cell body or axonal preparations, despite GAPDH showing more protein in those samples than in the Khsrp+/+ samples.