Table 1.
Single particle cryo-EM data collection, processing, and model building for E. coli Rob-TACII, Rob-TACI
| Rob-TACII | Rob-TACI | |
|---|---|---|
| Data collection and processing | ||
| Microscope | Titan Krios | Titan Krios |
| Voltage (kV) | 300 | 300 |
| Detector | K2 summit | K3 summit |
| Electron exposure (e/Å2) | 50 | 50 |
| Defocus range (μm) | 1.4–2.2 | 1.4–2.2 |
| Data collection mode | Count | Super resolution |
| Physical pixel size (Å/pixel) | 1.307 | 1.100 |
| Symmetry imposed | C1 | C1 |
| Initial particle images | 478 388 | 962 734 |
| Final particle images | 335 021 | 386 188 |
| Map resolution (Å)a | 4.57 | 4.06 |
| Refinement | ||
| Map sharpening B-factor (Å) | –89 | –101 |
| Root-mean-square deviation | ||
| Bond length (Å) | 0.006 | 0.007 |
| Bond angle (º) | 0.904 | 0.993 |
| MolProbity statistics | ||
| Clashscore | 12.00 | 10.00 |
| Rotamer outliers (%) | 1.10 | 1.00 |
| Cβ outliers (%) | 0.0 | 0.0 |
| Ramachandran plot | ||
| Favored (%) | 91.84 | 91.38 |
| Outliers (%) | 0.20 | 0.20 |
aGold-standard FSC 0.143 cutoff criteria.