Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 27;50(10):5652–5671. doi: 10.1093/nar/gkac408

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — top-3-ZnF and rmh-2 double mutants display a synthetic phenotype. (A) Representative images of gonads stained with HA (green) and DAPI (magenta) in the indicated genotypes. Zone 1 = transition zone; zone 2 = early pachynema; zone 3 = mid pachynema; zone 4 = late pachynema. Right panel: HA foci quantification in the indicated genotypes. The scatter plot indicates the number of foci per nucleus. Mean ± SD are indicated, n = number of nuclei assessed. top-3-ZnF::ha (same quantification as in Figure 3A): zone 1, 0.3 ± 0.6, n = 121; zone 2, 5.5 ± 5, n = 92; zone 3, 14.8 ± 4.1, n = 83; and zone 4, 7.8 ± 1.4, n = 63. top-3::ha; rmh-2: zone 1, 0.9 ± 0.9, n = 89; zone 2, 9.4 ± 6, n = 88; zone 3, 16.3 ± 5.4, n = 70; and zone 4, 7.4 ± 1.4, n = 48. **** P < 0.0001, as determined using the Mann–Whitney test; non-significant differences are not shown. top-3-Zn; rmh-2: no quantification owing to a lack of foci. (B) Percentage viability and rates of larval arrest and males in the indicated genotypes, with n = number of worms quantified per genotype: rmh-2(jf94); top-3-ZnF::han = 10; and top-3-ZnF = 17 (same as in Figure 1C); rmh-2(jf94)n = 9. (C) Quantification of DAPI bodies in the −1 diakinesis oocyte in the indicated genotypes: wt (same quantification as in Figure 1E) 6 ± 0, n = 50; top-3-ZnF, 5.96 ± 0.3, n = 154 (same quantification as in Figure 1E); rmh-2, 6.1 ± 0.3, n = 50; rmh-2; top-3-ZnF, 6.8 ± 1.2, n = 51; spo-11, 11.9 ± 0.3, n = 28; and spo-11; rmh-2; top-3-ZnF, 12.1 ± 0.3, n = 49. ** P = 0.0099, * P = 0.028, **** P < 0.0001, as determined using the Mann–Whitney test; non-significant differences are not shown. Insets show representative images of DAPI-stained diakinesis nuclei of the indicated genotypes. The dashed circles highlight fragments of DNA. (D) Representative images of RAD-51 foci localization at different stages of meiotic prophase I in the indicated genotypes. Gonads were stained with DAPI (cyan) and anti-RAD-51 antibodies (red).