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. 2022 Jan 21;50(10):5443–5466. doi: 10.1093/nar/gkac018
Time Flow rate (ml/min) %A %B Curve
Initial 0.80 95.0% 5.0%
0.30 0.80 95.0 5.0 6
3.30 0.80 85.0 15.0 6
3.80 0.80 5.0 95.0 1
4.50 0.80 95.0 5.0 1
5.00 0.80 95.0 5.0 1