Figure 2.

CD4⁺CD25⁺CD226^- Tregs Exhibit Increased CD25 Expression. Representative histograms show expression of cell surface markers on CD127^lo/- (lighter blue) and CD226^- CD4⁺CD25⁺FOXP3⁺ Tregs (darker blue), CD127^lo/- (lighter red) and CD226^- CD4⁺CD25⁺FOXP3⁺Helios⁺ Tregs (darker red), and CD127^lo/- (lighter green) and CD226^- CD4⁺CD25⁺FOXP3⁺Helios^- Tregs (darker green) with violin plots showing stain index (SI) fold change from FMO controls, (A–F) prior to expansion (n =12 biological with n = 2 technical replicates) and (G–L) following 14 days of ex vivo expansion (n = 5 biological with n = 2 technical replicates). (A, B, G, H) CD226, (C, D, I, J) CD127, (E, F, K, L) CD25. Significant P-values are reported on the figure for two-way ANOVA with Bonferroni correction for multiple comparisons of Treg isolation conditions from matched subjects.