Table 1.
Target | Clone | Fluorochrome | Vendor | Concentration | RRID |
---|---|---|---|---|---|
CD4 | SK3 | BV510 | BD Biosciences | 0.05 µg/mL | AB_2744424 |
CD8 | RPA-T8 | PE-CF594 | BD Biosciences | 0.10 µg/mL | AB_11154052 |
CD25 | BC96 | APC | BioLegend | 0.50 µg/mL | AB_314280 |
CD25 | BC96 | BV605 | BioLegend | 0.50 µg/mL | AB_11218989 |
CD39 | eBioA1 | APC | eBioscience | 0.50 µg/mL | AB_1963578 |
CD40L | 24-31 | APC-Cy7 | BioLegend | 0.50 µg/mL | AB_2076096 |
CD45RA | HI100 | BV605 | BioLegend | 0.10 µg/mL | AB_2563814 |
CD73 | AD2 | PE | BD Pharmingen | 0.50 µg/mL | AB_393561 |
CD127 | A019D5 | PE | BioLegend | 0.20 µg/mL | AB_1937251 |
CD197 (CCR7) | 2-L1-A | APC-R700 | BD Biosciences | 0.10 µg/mL | AB_2869856 |
CD226 | 11A8 | PE-Cy7 | BioLegend | 0.40 µg/mL | AB_2616645 |
CLTA-4 | L3D10 | PE-Cy7 | BioLegend | 0.50 µg/mL | AB_2563098 |
FOXP3 | 206D | Alexa Fluor 488 | BioLegend | 0.50 µg/mL | AB_430883 |
FOXP3 | 259D | Alexa Fluor 488 | BioLegend | 0.50 µg/mL | AB_430887 |
GITR | 621 | PE-Cy5 | BioLegend | 0.50 µg/mL | AB_2240646 |
Helios | 22F6 | Pacific Blue | BioLegend | 0.25µg/mL | AB_10690535 |
IL-10 | JES3-9D7 | BV421 | BioLegend | 0.08 µg/mL | AB_2632952 |
IL-17A | BL168 | BV605 | BioLegend | 0.12 µg/mL | AB_2563887 |
IFN-γ | 4S.B3 | BV570 | BioLegend | 0.10 µg/mL | AB_2563880 |
PD-1 | EH12.2H7 | Alexa Fluor 647 | BioLegend | 0.50 µg/mL | AB_940471 |
TGF-β1 | TW4-2F8 | Alexa Fluor 647 | BioLegend | 0.40 µg/mL | AB_2721298 |
TGF-β1 | FNLAP | PerCP-eFluor 710 | eBioscience | 0.50 µg/mL | AB_2573900 |
TNF | Mab11 | BV650 | BioLegend | 0.20 µg/mL | AB_2561355 |