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. 2022 May 26;13:873560. doi: 10.3389/fimmu.2022.873560

Table 1.

Antibodies used for flow cytometry.

Target Clone Fluorochrome Vendor Concentration RRID
CD4 SK3 BV510 BD Biosciences 0.05 µg/mL AB_2744424
CD8 RPA-T8 PE-CF594 BD Biosciences 0.10 µg/mL AB_11154052
CD25 BC96 APC BioLegend 0.50 µg/mL AB_314280
CD25 BC96 BV605 BioLegend 0.50 µg/mL AB_11218989
CD39 eBioA1 APC eBioscience 0.50 µg/mL AB_1963578
CD40L 24-31 APC-Cy7 BioLegend 0.50 µg/mL AB_2076096
CD45RA HI100 BV605 BioLegend 0.10 µg/mL AB_2563814
CD73 AD2 PE BD Pharmingen 0.50 µg/mL AB_393561
CD127 A019D5 PE BioLegend 0.20 µg/mL AB_1937251
CD197 (CCR7) 2-L1-A APC-R700 BD Biosciences 0.10 µg/mL AB_2869856
CD226 11A8 PE-Cy7 BioLegend 0.40 µg/mL AB_2616645
CLTA-4 L3D10 PE-Cy7 BioLegend 0.50 µg/mL AB_2563098
FOXP3 206D Alexa Fluor 488 BioLegend 0.50 µg/mL AB_430883
FOXP3 259D Alexa Fluor 488 BioLegend 0.50 µg/mL AB_430887
GITR 621 PE-Cy5 BioLegend 0.50 µg/mL AB_2240646
Helios 22F6 Pacific Blue BioLegend 0.25µg/mL AB_10690535
IL-10 JES3-9D7 BV421 BioLegend 0.08 µg/mL AB_2632952
IL-17A BL168 BV605 BioLegend 0.12 µg/mL AB_2563887
IFN-γ 4S.B3 BV570 BioLegend 0.10 µg/mL AB_2563880
PD-1 EH12.2H7 Alexa Fluor 647 BioLegend 0.50 µg/mL AB_940471
TGF-β1 TW4-2F8 Alexa Fluor 647 BioLegend 0.40 µg/mL AB_2721298
TGF-β1 FNLAP PerCP-eFluor 710 eBioscience 0.50 µg/mL AB_2573900
TNF Mab11 BV650 BioLegend 0.20 µg/mL AB_2561355