TABLE 3.
The mRNA expression of EMT-related factors in mIBECs with Gli1/Gli2 interference.
| Objects | Groups | Snail | Slug | E-cadherin | α-SMA | Vimentin |
| mIBECs | Normal control | 3.21 ± 1.21 | 2.40 ± 1.00 | 3.90 ± 1.33 | 3.12 ± 0.96 | 4.37 ± 1.46 |
| Lentivirus-empty | 5.21 ± 0.88 | 3.03 ± 0.87 | 4.36 ± 0.85 | 3.46 ± 1.24 | 3.67 ± 1.04 | |
| ADV-empty | 4.32 ± 0.78 | 2.63 ± 1.10 | 6.09 ± 1.40 | 4.09 ± 1.03 | 4.58 ± 0.95 | |
| Gli1-shRNA | 3.42 ± 0.80 | 2.25 ± 0.48 | 6.27 ± 3.53 | 4.19 ± 2.30 | 10.39 ± 4.42a | |
| ADV-Gli1 | 7.94 ± 2.49b | 3.51 ± 0.73 | 2.35 ± 1.10b | 8.40 ± 3.87b | 6.47 ± 1.78 | |
| Gli2-shRNA | 2.23 ± 0.67a | 1.68 ± 1.03 | 9.09 ± 1.29a | 2.74 ± 0.61 | 3.11 ± 1.07a | |
| ADV-Gli2 | 9.89 ± 2.06b | 3.74 ± 1.11 | 2.30 ± 0.39b | 12.41 ± 3.00b | 14.51 ± 3.63b |
ADV, adenovirus; mIBECs, mouse intrahepatic bile duct epithelial cells. Values are given as the mean ± SD.
Comparison of the GLI1/GLI2-shRNA and lentivirus-empty groups in mIBECs, aP < 0.05; comparison of the ADV-GLI1/GLI2 and ADV-empty groups in mIBECs, bP < 0.05.