TABLE 3.

Chondroitin sulfate A-, chondroitin sulfate C-, and dermatan sulfate-degrading activities in soluble extracts from E. coli strains containing expression plasmids^a

Temp (°C) and substrate	Avg activity (mIU ml−1A600 unit−1) ± SEb
	pIBX1	pIBX7	pIBX8
37
CS-Ac	<3.0	17.6 ± 3.3	NDf
CS-Cd	<3.0	11.7 ± 1.6	ND
DSe	<3.0	ND	13.7 ± 2.2
30
CS-A	<3.0	36.7 ± 0.6	ND
CS-C	<3.0	26.0 ± 0.5	ND
DS	<3.0	ND	21.7 ± 2.4
25
CS-A	<3.0	46.9 ± 3.0	ND
CS-C	<3.0	31.3 ± 1.4	ND
DS	<3.0	ND	62.5 ± 2.4

^aValues are based on three individual experiments. 

^bOne unit of chondroitin sulfate A-, chondroitin sulfate C-, or dermatan sulfate-degrading activity is defined as the amount of enzyme that will liberate 1 μmol of product per min at 30°C and pH 8. 

^cCS-A, chondroitin sulfate A. 

^dCS-C, chondroitin sulfate C. 

^eDS, dermatan sulfate. 

^fND, not determined.