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. 2022 May 26;13:881057. doi: 10.3389/fphar.2022.881057

FIGURE 4.

FIGURE 4

Effects of TFRD on gene expression levels of BMP-2/Runx2 and OPG/RANKL in tibial growth plate in TD broilers. (A–D) Principal component analysis (PCA) of the BMP-2, Runx2, OPG and RANKL gene expressions with an unsupervised pattern recognition method. (E) The mRNA expression of genes BMP-2, Runx2, OPG, RANKL and the ratio of OPG/RANKL at day 7, 14 and 21. (F) The heat map shows the mRNA levels of the bone-related genes. The results are represented as mean ± SD. a, b, c, and d represent significant differences between groups (p < 0.05).