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Coomassie blue-stained gel after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of extracts of L. lactis NZ9000 harboring pNZ8136, which contains MG1363 metC (lanes 1 [uninduced] and 2 [induced], or harboring pNZ8137, which contains B78 metC (lane 3 [induced]). The cultures were induced with 2.5 ng of nisin per ml. Lane M, molecular weight markers. Molecular weights (in thousands) are noted at the left. The location of the overproduced proteins is indicated (arrow).
