TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid(s) | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| E. coli MC1061 | 7 | |
| L. lactis | ||
| MG1363 | Plasmid free | 24 |
| NIZO B78 | Starter strain | NIZO collection |
| NZ9000 | MG1363 pepN::nisRK | 31 |
| MG1363ΔmetC | MG1363 derivative, ΔmetC | This study |
| Plasmids | ||
| pUC18, pUC19 | Ampr | 45 |
| pNZ8133 | Ampr, 4.8-kb pUC18 derivative carrying a 2.2-kb HindIII-PstI fragment from L. lactis MG1363 | This study |
| pNZ8134 | Ampr, 6.8-kb pUC19 derivative carrying a 4.2-kb EcoRI fragment from L. lactis MG1363 | This study |
| pNZ8135 | Ampr, 3.8-kb pUC19 derivative carrying a 1.2-kb BamHI-SalI fragment of the metC gene from L. lactis B78 | This study |
| pNZ8037 | Cmr, 3.4-kb, carrying the nisA promoter and MCS | 14 |
| pNZ8136 | Cmr, 4.5-kb pNZ8048 derivative carrying the MG1363 metC gene | This study |
| pNZ8137 | Cmr, 4.5-kb pNZ8048 derivative carrying the B78 metC gene | This study |
| pG+host8 | Tetr, 4.3-kb pWV01 thermosensitive replicon | 34 |
| pNZ8138 | Tetr, 4.9-kb pG+host8 derivative carrying a 665-bp internal fragment of the MG1363 metC gene | This study |
| pNZ9530 | Eryr, 7.0-kb pIL252 derivative containing nisR and nisK genes | 29 |
a
Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Eryr, erythromycin resistant; Tetr, tetracycline resistant; MCS, multiple cloning sites.