Figure 5.

The regulation of the transcription and expression of ACSL4 by YAP entry into the nucleus in primary myoblasts. (A) KEGG analyses of RNA‐seq data showing the top 10 enriched pathways in EHS mice compared with SHR mice. (B–C) The location of YAP in nuclear was determined by laser scanning confocal microscopy (n = 6). (D–E) In parallel, QPCR analysis of Acsl4 and Yap mRNA levels in primary myoblast subjected to io + hs and Gas muscle tissues of mice with EHS, samples were collected at 6 h after EHS onset or after io + hs induction during recovery time (n = 6 mice/group or n = 6). (F–K) Primary myoblast cells were transfected with Yap overexpression plasmid or siRNA for 2 days, and then subjected to io + hs induction. The YAP protein levels and the mRNA and protein levels of ACSL4 were determined after io + hs induction. The representative western blotting images were from three independent experiments. Summary data are presented as the mean ± SEM. Significance was calculated using a one‐way ANOVA with Tukey's post hoc test; groups labelled with different letters differed significantly (*P < 0.05).