Figure 6.

Treatment with CXCR4 antagonist reduces BM inflammation in MKO mice. (A) G‐MAD analysis shows that CXCR4 is associated with T‐cell migration and proliferation, and with chemokine‐binding modules, in mice. The threshold of significant gene‐module association is indicated by the red dashed line. Modules are organized by module similarities. Known modules connected to CXCR4 are highlighted in red. GMAS, gene‐module association score. (B) BM cells were subjected to flow cytometry for mature T‐cells, natural killer, and natural killer T‐cell phenotypes. (C,D) Populations of BM CD44⁺IFN‐γ⁺ or CD44⁺TNF‐α⁺ among CD4⁺ T‐cells from control and MKO mice treated with AMD3100 or vehicle at 14 weeks of age. (E) IL‐17A production by BM CD4⁺ T‐cells was analysed by flow cytometry. (F,G) Populations of BM CD44⁺IFN‐γ⁺ or CD44⁺TNF‐α⁺ among CD8⁺ T‐cells from control and MKO mice treated with AMD3100 or vehicle at 14 weeks of age. (H) Production of IFN‐γ or TNF‐α by BM CD4⁺CD44⁺ or CD8⁺CD44⁺ T‐cells of control and MKO mice treated with AMD3100 or vehicle at 14 weeks of age. Data are expressed as the mean ± standard error of the mean. Statistical significance was analysed by one‐way ANOVA.