Figure 6.

Impact of MyoMed‐205 on mitochondrial protein synthesis and enzyme activity in TA muscle. Protein synthesis of mitochondrial proteins or enzyme activities (A–E) were quantified by western blot analysis or specific enzyme assays in TA muscle homogenates obtained from ZSF1‐lean (lean), ZSF1 obese (obese) and ZSF1‐obese rats treated with MyoMed‐205 (obese + MyoMed‐205). Porin (A), mitochondrial complex I (C), mitochondrial complex II (D), mitochondrial complex III (E), mitochondrial complex IV (F), and mitochondrial complex V (G) synthesis were assessed by western blot and mitochondrial complex I enzyme activity was determined (B). Moreover, the enzymatic activity of citrate synthase (CS) in TA (I) and the protein synthesis of UCP3 was quantified (J). Results are expressed as mean ± SEM (n = 10–14 per group). A representative western blot shows the detection of mitochondrial complex I–V (H).