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The passages of HUC-MSCs without affecting the characteristics of stem cells. (a) Proliferation curve of HUC-MSCs. There were little changes in the proliferation rate of HUC-MSCs from passage 4 to passage 19. (b) Cellular senescence assay of different passages of HUC-MSCs. (c) Cell cycle of different passages of HUC-MSCs. (d) Apoptosis analysis of different passages of HUC-MSCs. (e) Identification of MSC markers for the 20th-generation HUC-MSCs by flow cytometry.
