Fig. 1.

rhMG53 localizes to the intercalated disc and mitochondria in mouse and pig cardiomyocytes after MI (A) Immunofluorescence (IF) of mg53 −/− mouse hearts after MI injury showed no MG53 signal in the saline treatment. Treatment with 2 mg/kg rhMG53 and staining of the left ventricle for MG53 (red), COX IV (green), and DAPI (blue) reveals co-localization of MG53 with mitochondria. White arrow indicates area of MG53 and COX4 co-localization. (B) IF of pig hearts treated with 2 mg/kg rhMG53 with MI injury highlights MG53 localization at the intercalated discs with a translocation to the mitochondria after MI injury. (C) Intensity analysis along the indicated grey line in part C shows an over-lapping striated pattern of MG53 and COX IV in pig heart with MI injury. White scale bar = 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)