FIG. 2.

Localization of the replication and stability regions. Construction of pMG101 derivatives is described in Table 1. The heavy horizontal lines indicate regions of plasmid pMG101 that were inserted into pHSG298. Replication was considered positive (+) when transformants were present and plasmid DNA was detected after alkaline lysis and gel electrophoresis and negative (−) when no transformants were obtained in three experiments. Plasmid stability in R. palustris was analyzed as described in Materials and Methods.