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. 2022 Apr 26;15(4):1087–1096. doi: 10.14202/vetworld.2022.1087-1096

Table 1.

The primer of Staphylococcal chromosomal mec typing.

Primer Sequence (5’–3’) Size of PCR product (bp) References
au-F3 TCGCTTGCTATGATTGTGG 359 [22]
au-R GCCAATGTTCTACCATAGC
in-F CATGTCATATTATTGCGAATGA 430
in-R3 AGGACCATCACCATTGACATATTGAAACC
sch-F AATGGCTACAATGATAATCACTAA 526
sch-R CATATCTGTCTTTCGGCGCG
hy-F1 CATTATATGATTTGAACGTG 793
hy-R1 GAATCAATATCGTAAAGTTGC
pse-F2 TRGGCAGTAGGATTCGTTAA 926
pse-R5 CTTTTGTGCTYCMTTTTGG
mecA-F AAAATCGATGGTAAAGGTTGGC 532 [23]
mecA-R AGTTCTGCAGTACCGGATTTGC
blaZ-F ACTTCAACACCTGCTGCTTTC 173 [24]
blaZ-R TGACCACTTTTATCAGCAACC
M-PCR 1 (for amplification of ccr gene complex with mecA) [25]
mA1 GCTATCCACCCTCAAACAGG 286
mA2 ACGTTGTAACCACCCCAAGA
α1 AACCTATATCATCAATCAGTACGT 695
α2 TAAAGGCATCAATGCACAAACACT 937
α3 AGCTCAAAAGCAAGCAATAGAAT 1791
βc ATTGCCTTGATAATAGCCITCT 1287
α4.2 GTATCAATGCACCAGAACTT
β4.2 TTGCGACTCTCTTGGCGTTT
γR CGTCTATTACAAGATGTTAAGGATAAT 518
γF CCTTTATAGACTGGATTATTCAAAATAT
M-PCR 2 (for amplification of mec gene complex class)
mI6 CATAACTTCCCATTCTGCAGATG 1963
IS7 ATGCTTAATGATAGCATCCGAATG 2827
IS2 TGAGGTTATTCAGATATTTCGATGT
mA7 ATATACCAAACCCGACAACTACA 804

M-PCR=Multiplex polymerase chain reaction