Table 1.
Primer | Sequence (5’–3’) | Size of PCR product (bp) | References |
---|---|---|---|
au-F3 | TCGCTTGCTATGATTGTGG | 359 | [22] |
au-R | GCCAATGTTCTACCATAGC | ||
in-F | CATGTCATATTATTGCGAATGA | 430 | |
in-R3 | AGGACCATCACCATTGACATATTGAAACC | ||
sch-F | AATGGCTACAATGATAATCACTAA | 526 | |
sch-R | CATATCTGTCTTTCGGCGCG | ||
hy-F1 | CATTATATGATTTGAACGTG | 793 | |
hy-R1 | GAATCAATATCGTAAAGTTGC | ||
pse-F2 | TRGGCAGTAGGATTCGTTAA | 926 | |
pse-R5 | CTTTTGTGCTYCMTTTTGG | ||
mecA-F | AAAATCGATGGTAAAGGTTGGC | 532 | [23] |
mecA-R | AGTTCTGCAGTACCGGATTTGC | ||
blaZ-F | ACTTCAACACCTGCTGCTTTC | 173 | [24] |
blaZ-R | TGACCACTTTTATCAGCAACC | ||
M-PCR 1 (for amplification of ccr gene complex with mecA) | [25] | ||
mA1 | GCTATCCACCCTCAAACAGG | 286 | |
mA2 | ACGTTGTAACCACCCCAAGA | ||
α1 | AACCTATATCATCAATCAGTACGT | 695 | |
α2 | TAAAGGCATCAATGCACAAACACT | 937 | |
α3 | AGCTCAAAAGCAAGCAATAGAAT | 1791 | |
βc | ATTGCCTTGATAATAGCCITCT | 1287 | |
α4.2 | GTATCAATGCACCAGAACTT | ||
β4.2 | TTGCGACTCTCTTGGCGTTT | ||
γR | CGTCTATTACAAGATGTTAAGGATAAT | 518 | |
γF | CCTTTATAGACTGGATTATTCAAAATAT | ||
M-PCR 2 (for amplification of mec gene complex class) | |||
mI6 | CATAACTTCCCATTCTGCAGATG | 1963 | |
IS7 | ATGCTTAATGATAGCATCCGAATG | 2827 | |
IS2 | TGAGGTTATTCAGATATTTCGATGT | ||
mA7 | ATATACCAAACCCGACAACTACA | 804 |
M-PCR=Multiplex polymerase chain reaction