Table I.
Gene | Forward primer, 5′-3′ | Reverse primer, 5′-3′ |
---|---|---|
IFI16 | CTCGGAGAGCTCGGACAG | TACCTATGACGACGCTGCTG |
ISG15 | GCCTCAGCTCTGACACC | CGAACTCATCTTTGCCAGTACA |
IFNB1 | TCTGGCACAACAGGTAGTAGGC | GAGAAGCACAACAGGAGAGCAA |
DNMT1 | CAGCAACGGGCAGATGTTTC | CGGAGGGTGCTTTGTAGATG |
DNMT3a | CTACGCACCACCTCCACCAG | CAATGTTCCGGCACTTCTGC |
DNMT3b | GAGTCCATTGCTGTTGGAACCG | ATGTCCCTTTGTCGCCAACCT |
Methylated IFI16 | TTCGAGTAGTTGGGATTATAGGC | TAATACAAAATTAACTAAACGCGAT |
Unmethylated IFI16 | TTTTTTGAGTAGTTGGGATTATAGGT | AAATAATACAAAATTAACTAAACACAAT |
IFI, IFN-induced protein; ISG, IFN-stimulated gene; DNMT, DNA methyltransferase.