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. 2022 May 16;24(1):210. doi: 10.3892/ol.2022.13331

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Knockdown of ApoM promotes SREBF1 to regulate PFKL to promote the proliferation of hepatoma cells. An EDU staining assay was used to detect the proliferation activity of (A) Huh-7 cells or (B) Mhcc97h cells in each group (pcDNA3.1-NC, pcDNA3.1-SREBF1, si-NC, si-SREBF1, si-SREBF1+pcDNA3.1-PFKL and si-ApoM+si-SREBF1; scale bar, 100 µm). Statistical analysis of the results for (C) Huh-7 cells or (D) Mhcc97h cells using ImageJ. Each group was set up three times in parallel. ns, no significance; ***P<0.01 vs. NC. NC, negative control; SREBF1, sterol regulatory element-binding protein 1; siRNA, small interfering RNA; PFKL, ATP-dependent 6-phosphofructokinase, liver type; ApoM, apolipoprotein M.