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. 2022 May 16;24(1):210. doi: 10.3892/ol.2022.13331

Figure 4.

Figure 4.

Knockdown of ApoM promotes SREBF1 to regulate PFKL to promote the migration and invasion of hepatoma cells. Huh-7 cells or Mhcc97h cells were transfected with pcDNA3.1-NC, pcDNA3.1-SREBF1, si-NC, si-SREBF1, si-SREBF1 + pcDNA3.1-PFKL or si-ApoM + si-SREBF1. (A-H) Transwell assays. Representative images of Huh-7 cells transgressed through the membrane in the (A) migration and (B) invasion experiment and quantified results for (C) migration and (D) invasion. Representative images of Mhcc97h cells transgressed through the membrane in the (E) migration and (F) invasion experiment and quantified results for (G) migration and (H) invasion (scale bars, 100 µm). (I-L) Wound-healing assay. (I) Representative images of migration of Huh-7 cells and (J) quantified results. (K) Representative images of migration of Mhcc97h cells (scale bars, 200 µm) and (L) quantified results. Each group was set up three times in parallel. ns, no significance; ***P<0.01 vs. NC. NC, negative control; SREBF1, sterol regulatory element-binding protein 1; si-, small interfering RNA; PFKL, ATP-dependent 6-phosphofructokinase, liver type; ApoM, apolipoprotein M.