TABLE 1.
Media used for detection of hyperthermophiles from the Kubiki oil reservoir
| Mediuma | Gas phase | Carbon source |
|---|---|---|
| HC | H2:CO2 (4:1,200 kPa) | Free |
| HA | H2:CO2 (4:1,200 kPa) | Acetate (0.1%) + lactate (0.1%) |
| AY | Air (100 kPa) | Yeast extract (0.2%) |
| YE | N2 (100 kPa) | Yeast extract (0.2%) |
| AA | N2 (100 kPa) | 20 amino acidsb (total, 0.2%) |
| GS | N2 (100 kPa) | Glucose (0.1%) + starch (0.1%) |
| AL | N2 (100 kPa) | Acetate (0.1%) + lactate (0.1%) |
| CO | N2 (100 kPa) | Crude oilc (10% [vol/vol]) |
a
Media based on artificial seawater were incubated for 1 to 7 days at 85°C in a dry oven.
b
The amino acids Gly, Ala, Ser, Thr, Cys, Asn, Gln, Leu, Ile, Val, Met, Phe, Tyr, Trp, Pro, Asp, Glu, His, Lys, and Arg were added at a final concentration of 0.01% (wt/vol) each.
c
Crude oil was collected from the no. 3 storage tank and autoclaved at 121°C for 30 min.