Fig. 3.

Knock-down of CBX2 induces apoptosis of AML cells. A Annexin V percentage of shCBX2- and shSCR-transduced cells; experiments were performed at 7 days of puromycin selection; error bars show SD of three biological replicates (*P < 0.05, **P ≤ 0.01). B WB analysis of the indicated proteins in shCBX2- and shSCR-transduced U937 cells at 7 days of puromycin selection. ERK1/2 was used as loading control; immunoblots were performed sequentially on the same membrane. C PI analysis of ex vivo AML blasts (from three different patients) knocked-down for CBX2. Experiments were performed at 3 days of puromycin selection; error bars show SD of two biological replicates