Fig. 4.

CBX2 silencing opens new chromatin regions in AML. A Venn diagram of ATAC-seq unique and shared peaks under shSCR and CBX2 knock-down conditions. B Heatmap analysis of ATAC-seq experiments showing shSCR and shCBX2 unique as well as shared regions in U937 cells; co-occurrence of GFP-CBX2, GFP and H3K27me3 occupancy at the ATAC-seq newly identified sites in the indicated cell lines. C Peak intensity quantification of shSCR, shCBX2, GFP negative control, GFP-CBX2 and H3K27me3 samples. D ATAC-seq examples of shCBX2 and shSCR samples together with GFP-CBX2, GFP and H3K27me3 occupancy at the indicated genomic locus. E Pathway analysis of shCBX2-associated unique regions; the top 5 significant enriched pathways are shown. F Genomic distance of shCBX2 unique, shSCR unique and shared ATAC-seq peaks from transcriptional start site. G Enrichment motif analysis of shCBX2 unique compared to shared sites