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Effect of DHEA on Smad2/3 and Akt activation. Western blot analyses of P-Smad2/Smad2 (A), P-Smad3/Smad3 (B) and P-Akt/Akt ratio (C) in normal human lung fibroblasts treated for one hour with DHEA 150 µM (D150) and/or TGF-β1 (5 ng/ml) (T + D150). Bars show mean ± SD. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001. D150: dehydroepiandrosterone 150 µM; RM: resting medium; SD: standard deviation; TGF-β1: transforming growth factor β1, T + D150: TGF- β1 + DHEA 150 µM
