Fig. 7.

Sex ratio distortion in PRSSLY mutant offspring. A Structure of mouse Prssly gene. Exons are indicated by boxes, and introns by lines; both drawn to scale. Conserved trypsin-like serine protease domains are shaded blue. Arrows indicate positions of CRISPR guide RNAs. B and C Total number of male and female offspring in all four mutant lines vs. controls (B) and number of male and female offspring in each mutant line (C). Two-sided chi-square tests comparing offspring sex numbers in mutants (observed) vs. controls (expected) were performed; p=0.019 for all mutants vs. controls (B) and p=0.043 for Δ47 (C). P values for Δ407, Δ14, and ins289 were not significant