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CO-binding assaya of various E. coli MG1655-expressed hemoglobin proteins
| Plasmid | Hemoglobin | Absorption value (nm) for activity
|
|
|---|---|---|---|
| Maximum | Minimum | ||
| pPPC1 | VHb | 419 | 437 |
| pAX1 | FHPg | 422 | 437 |
| pAX4 | VHb-FAD-NAD | 422 | 443 |
| pAX5 | FHP | 422 | 439 |
| pAX9 | VHb-FAD | 419 | 436 |
| pAX6 | FHPg-FAD | 421 | 438 |
| pAX12 | VHb-NAD | 416 | 442 |
| pAX14 | FHPg-NAD | 419 | 436 |
Performed as described in reference 13.
