Figure 1.

OX2R agonist OB-Ala reduced energy expenditure and food intake.

Mice received daily i.p. injection of OX2R agonist OB-Ala (16 nmol/kg) or saline for 3 weeks. Metabolic parameters were obtained after 3 weeks of treatment. A and B: Bodyweight (A) and average daily food intake (B) of mice. C: Rectal temperature of mice. D and E: Diastolic pressure (D) and systolic pressure (E) of mice. F–H: Heat production during the dark phase (F), the light phase (G), and in total (H) of mice was measured by the metabolic cages. I: Total running distance of mice measured by the metabolic cages. ^*P<0.05.n=7 for saline;n=9 for OB-Ala. Data arepresented as mean±SEM. Statistical significance was determined by unpaired t-test. OX2R: orexin receptor type 2; OB-Ala: [Ala11, D-Leu15]-OxB.