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. 2022 May 28;36(3):195–207. doi: 10.7555/JBR.36.20220033

Figure 3.

Figure 3

OX2R is selectively expressed in brown adipose tissue and does not affect white adipose tissue.

Mice received daily i.p. injection of OX2R agonist OB-Ala (16 nmol/kg) or saline for 3 weeks. iWAT was analyzed after 3 weeks of treatment. A: Representative images of H&E staining of iWAT. B: Sectional area of iWAT adipocytes,n=7 for saline;n=9 for OB-Ala. C: Relative gene expression of Adrb3, Lpl, Hsl, and Cfd of iWAT. n=7 for saline;n=9 for OB-Ala. Rps18 was used as the internalreference. D: OX2R protein levels in iWAT, iBAT, PVAD and PAT analyzed by Western blotting assay. Scale bar = 25 μm (A). Data are presented as mean±SEM. Statistical significance was determined by unpaired t-test. OX2R: orexin receptor type 2; iWAT: inguinal white adipose tissue; iBAT: intrascapular brown adipose tissue; PVAD: perivascular adipose tissue; PAT: pericardial adipose tissue.