Figure 7.

Intra-BAT infusion of OB-Ala inhibited iBAT thermogenesis during cold exposure.

Mice were subjected to cold exposure in a 4 °C chamber for one hour after microinjection of OB-Ala (0.3 nmol) into iBAT. A: iBAT temperature of mice upon cold exposure challenge. B: Change of rectal temperature of mice after cold exposure. C: c-Fos levels in PVN of mice after cold exposure. D: Quantification of (C). E: c-Fos levels in DMH of mice after cold exposure. F: Quantification of (E). n=6 for saline; n=7 for OB-Ala. ^**P<0.01,^***P<0.001. Data arepresented as mean±SEM. Statistical significance was determined by Two-way ANOVA (A) or unpairedt-test (B, D, and F). Scale bar: 50 μm (C and E). PVN: paraventricular nucleus; DMH: dorsal medial hypothalamus; III: third ventricle; Tbat: temperature of brown adipose tissue; T-rectal: rectal temperature.