Figure 1.

rBoNT/A1 ameliorates mechanical allodynia in CFA-evoked local hind paw inflammation in mice. (A) Schematic representation of the experimental protocol used for in vivo calcium imaging, behavioral and histology experiments. CFA and rBoNT/A1 or vehicle (gelatine phosphate buffer; GPB) were injected on day -2 and day 0, respectively, followed by behavior measurements on day 3 and 5. In vivo calcium imaging was performed on day 3. Recordings at baseline were followed by mechanical and thermal stimulation. On day 5, tissues were collected for histological analysis. (B) Mechanical allodynia (g) was measured by von Frey filaments. Two-way ANOVA, post hoc Tukey. *(within groups to baseline) and §(across groups): p < 0.05. N = 10/group.