Skip to main content
. 2022 May 26;15:909835. doi: 10.3389/fnmol.2022.909835

Figure 7.

Figure 7

rBoNT/A1 fails to inhibit calcium transients in the DRG upon peripheral heat stimulation. (A) Schematic drawing of the thermal stimulation protocol in line with traces below depicting calcium transients normalize to baseline (ΔFoF) in individual neurons from mice treated with CFA and rBoNT/A1 (top), CFA and GPB (vehicle; middle), or vehicle alone (bottom) over time. Baseline temperature was set to 32°C. To apply stimuli, heat was increased to 40°C, 45°C, and 50°C for 8 s each. In-between the stimuli, temperature returned to baseline. (B) False color images of GCaMP6s-labeled DRG neurons in situ at baseline and after noxious heat stimulation (50°C) from mice treated with CFA and rBoNT/A1 or CFA and vehicle (GPB) or vehicle alone. (C) Percentage of neurons responding to noxious heat under conditions as indicated. (D) Magnitude of responses upon heat stimulation with 40°C, 45°C, and 50°C in treatment groups as indicated. N = 4 for CFA/rBoNT/A1, n = 5 for CFA/vehicle and n = 5 for control (PBS). Mean ± SEM. Mixed-effect analysis, post hoc Tukey. p < 0.05. *(across groups): CFA plus rBoNT/A1 or vehicle vs. control; §(within groups) compared to 40°C.