TABLE 3.
Effect of UV irradiation on the incorporation of 14C in D. radioduransa
| Growth medium | UV treatment | Total cpm accumulated per 108 cells | Total cpm found in DNA extracted per 108 cells | % Of 14C incorporated into DNA per cell |
|---|---|---|---|---|
| No | 5,186.5 | 414.3 | 8.0 | |
| TGY | Yes | 2,789.6 | 512.4 | 18.4 |
| No | 8,544.5 | 327.2 | 3.8 | |
| TGY+Mn | Yes | 8,009.1 | 474.6 | 5.9 |
a
Mid-log-phase cells were transferred to flasks containing 5 ml of fresh TGY medium plus 2 μCi of d-glucose-UL-14C with or without Mn. After 2 h of incubation at 30°C, half of each sample was illuminated with UV light for 5 h. The remaining half of each sample was incubated without UV treatment and was used as the control. Standard procedure was used for DNA extraction. Results were the mean values from triplicate samples (standard derivation was less than 2%).