Mouse gut microbial alpha diversity across time points. Alpha diversity metrics measured at T1 (day 5—post-FMT), T2 (day 13—post tumor cell injection), T3 (day 22—post paclitaxel), and T4 (day 27/28—euthanasia) and compared between groups using Kruskal-Wallis pairwise tests (A-C). Observed Species did not differ significantly between groups (A). Evenness differed significantly between groups at each timepoint (B). Shannon diversity differed significantly between groups at T2 (day 13) (C). * Indicates a significant difference (p < 0.05). Data are presented as ± SEM and each dot represents an individual mouse’s results. BCW0 (n = 10), BCW12 (n = 12), BCW12OFS (n = 12).