Figure 3.

Epigenetic DNA modification-related gene expression analysis in treatment-responsive and -refractory AML patients. Cell samples were collected at diagnosis stage and after treatment. Relative gene expression analysis was performed the using RT-qPCR ΔΔCt method; GAPDH was used as a “housekeeping” gene. Mean ± standard deviation is presented; grey data points indicate outliers. Mann–Whitney U test was used to determine the significance of difference between groups of different patients’ samples, and significance was set at p ≤ 0.05 (*). Outliers were determined by ROUT (Q = 5%).