Behavioral analysis of pooled WT mice and young and old GLA KO mice including von Frey test, Hargreaves test, and cold sensitivity test. (A) Von Frey testing of pooled WT mice (△, n = 38) and young GLA KO mice (▲, n = 25). (B) Von Frey testing of pooled WT mice (◯, n = 38) and old GLA KO mice (●, n = 15). (C) Hargreaves testing of pooled WT mice (△, n = 43) and young GLA KO mice (▲, n = 24). (D) Hargreaves testing of young (▲, n = 24) and old (●, n = 14) GLA KO mice. (E) Hargreaves testing of pooled WT mice (◯, n = 43) and old GLA KO mice (●, n = 14). (F) Cold sensitivity testing of pooled WT mice (△, n = 37) and young GLA KO mice (▲, n = 21). (G) Cold sensitivity testing of pooled WT mice (◯, n = 37) and old GLA KO mice (●, n = 15). Abbreviations: g = gram; GLA KO = alpha-galactosidase A knockout; sec = seconds; WT = wildtype. ** p < 0.01, *** p < 0.001.